5-Formylcytosines (5-fC) DNA Matched Antibody Pair
Product Description
This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
RM476 Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody
Product Data Sheet
5-Formylcytosines (5-fC) DNA Matched Antibody Pair Datasheet
Specificity
This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA.
1) Capture Antibody: Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.
2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
Purity
Protein A affinity purified from an animal origin–free culture supernatant
Detection of 5-Formylcytosines (5-fC) in linear denatured DNA (426 bp). Sandwich
ELISA using anti-Formylcytosines (5-fC) (clone RM477) as the capture antibody
(50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476)
as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.