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GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves the arrest of the progenitor cell cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggest that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same. Recombinant Human/Murine/Rat GDF-11 is a 25.0 kDa disulfide-linked homodimer containing two 109 amino acid polypeptide chains. It is highly homologous to myostatin/GDF-8, sharing 90% amino acid sequence identity.

Source: E.coli

Synonyms: Growth/Differentiation Factor-11, BMP-11

AA Sequence (monomer): NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Assay #1: Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED₅₀ for this effect is 0.08-0.10 μg/ml.

Assay #2:?Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1cells. The ED₅₀ for this effect is 8-10 ng/ml.

Note:

1mg will be provided as 2x500μg.